Project

Part:BBa_K525002

Designed by: Anna Drong   Group: iGEM11_Bielefeld-Germany   (2011-09-11)
Revision as of 13:01, 11 September 2011 by Registry (Talk | contribs)

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Fusion protein of SbpA, NADP+ Oxidoreductase, BisdA and BisdB with PT7/lac

PT7 + RBS + SbpA || Red || BisdA || BisdB


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 104
    Illegal BglII site found at 221
    Illegal BamHI site found at 4539
    Illegal BamHI site found at 5277
    Illegal XhoI site found at 1996
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 76
    Illegal AgeI site found at 5530
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 493
    Illegal BsaI.rc site found at 622
    Illegal BsaI.rc site found at 3874


[edit]
Categories
//chassis/prokaryote/ecoli
//function/degradation/bisphenol
//proteindomain/degradation
//proteindomain/internal
//rnap/bacteriophage/t7
Parameters
biologyS-Layer
chassisE. coli
originLysinibacillus sphaericus, Escherichia coli, Sphingomonas bisphenolicum AO1