Translational_Unit

Part:BBa_K515000

Designed by: Atipat Patharagulpong   Group: iGEM11_Imperial_College_London   (2011-09-01)
Revision as of 14:51, 10 September 2011 by Nikkikapp (Talk | contribs)

IaaM - tryptophan-2-mono-oxygenase

IAA tryptophan monooxygenase (IaaM) catalyzes the oxidative carboxylation of L-tryptophan to indole-3-acetamide which acts as an intermediate to the production of indole-3-acetic acid in the IAM pathway.[1] There are several different pathways to produce indole-3-acetic acid. [2] We are using genes from the IAM pathway (IaaM and IaaH) of the plant pathogen, Psuedomonas savastanoi to produce indole-3-acetic acid in E. coli </p>

tryptophan --> indole acetamide --> indole-3-acetic acid


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 450
    Illegal BamHI site found at 1395
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 157
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1]http://ecocyc.org/META/NEW-IMAGE?type=GENE&object=G-5821 [2]Spaepen S. et al., 2007. Indole-3-acetic acid in microbial and microorganism-plant signaling. Federation of European Microbiological Societies Microbiology Reviews , 31, pp.425–448 </p>

[edit]
Categories
//cds/enzyme
Parameters
chassisE. coli DH5α
device_typepathway
input_stryptophan
originP. savastanoi
outputIndole acetamide
resistancechloramphenicol