Translational_Unit

Part:BBa_K515000

Designed by: Atipat Patharagulpong   Group: iGEM11_Imperial_College_London   (2011-09-01)
Revision as of 12:34, 9 September 2011 by Nikkikapp (Talk | contribs)

IaaM - tryptophan-2-mono-oxygenase

IAA tryptophan monooxygenase catalyzes the oxidative carboxylation of L-tryptophan to indole-3-acetamide. The enzymes of the IAM pathway (IaaM and IaaH) originate from the plant pathogen, Psuedomonas savastanoi which produces and secretes indole-3-acetic acid (auxin).

The sequence has been codon optimised for E. coli and B. subtilis and is under control of the pVEG promoter with an RBS also suitable for E. coli and B. subtilis.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 450
    Illegal BamHI site found at 1395
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 157
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/enzyme
Parameters
chassisE. coli DH5α
device_typepathway
input_stryptophan
originP. savastanoi
outputIndole acetamide
resistancechloramphenicol