Translational_Unit

Part:BBa_K515000

Designed by: Atipat Patharagulpong   Group: iGEM11_Imperial_College_London   (2011-09-01)
Revision as of 11:08, 9 September 2011 by Nikkikapp (Talk | contribs)

IaaM - tryptophan-2-mono-oxygenase

IaaM encodes IAA tryptophan monooxygenase which converts tryptophan to indoleacetamide. The sequence for this is from Psuedomonas savastanoi which uses a two-enzyme pathway to produce indole 3 acetic acid (auxin).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 450
    Illegal BamHI site found at 1395
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 157
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/enzyme
Parameters
chassisE. coli DH5α
device_typepathway
input_stryptophan
originP. savastanoi
outputIndole acetamide
resistancechloramphenicol