Coding

Part:BBa_K523013

Designed by: Sylvia Ispasanie, Mun Ching Lee   Group: iGEM11_Edinburgh   (2011-09-05)
Revision as of 09:46, 6 September 2011 by Allancrossman (Talk | contribs)

Plac + INP-EYFP

Fusion of Ice Nucleation Protein (INP) and Enhanced Yellow Fluorescent Protein (EYFP) under the control of the Lac promoter.

Constructed using the BioSandwich protocol of Edinburgh 2011.

Usage and Biology

Edinburgh-INP-YFP-cells.jpg

Cells expressing this construct are shown on the right. The INP should carry the EYFP to the outer membrane of E. coli. Our imaging technology was not good enough to confirm this.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1036
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None