Coding

Part:BBa_K523013

Designed by: Sylvia Ispasanie, Mun Ching Lee   Group: iGEM11_Edinburgh   (2011-09-05)
Revision as of 09:51, 5 September 2011 by Allancrossman (Talk | contribs)

Plac + INP-EYFP

Fusion of Ice Nucleation Protein (INP) and Enhanced Yellow Fluorescent Protein (EYFP) under the control of the Lac promoter.

The BioSandwich assembly method places short (~40 b.p.) spacers between parts.


Usage and Biology

The INP should carry the EYFP to the outer membrane of E. coli.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1036
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None