Plasmid

Part:BBa_K626000

Designed by: Endre Károly Kristóf   Group: iGEM11_Debrecen_Hungary   (2011-09-01)
Revision as of 14:59, 1 September 2011 by Mrtwister (Talk | contribs)

TRE-Bax-PolyA in PSB1A3 (expression vector)

Expression vector from standard BioBrick parts, expressing human pro-apoptotic protein BAX.

The human BAX is a pro-apoptotic protein which can be expressed by this expression vector.

The minimal CMV promoter ensures a continuous expression of the protein, but furthermore it can be expressed in an inducible form using the Tetracyclin-controlled transcriptional activation system.

hBAX

Bax (Bcl-2-associated X) protein is a pro-apoptotic member of the Bcl-2 protein family. It contains three BH (Bcl-2 homology) domains, can form homo- and heterodimers and plays essential role in the formation of MOMP (Mitochondrial outer membrane permeabilization) leading to apoptosis through cytochrome c release which is the most frequent type of PCD (Programmed cell death).



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 778
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None