Plasmid
Part:BBa_K626000
Designed by: Endre Károly Kristóf Group: iGEM11_Debrecen_Hungary (2011-09-01)
TRE-Bax-PolyA in PSB1A3 (expression vector)
Expression vector from standard BioBrick parts, expressing human pro-apoptotic protein BAX.
The human BAX is a pro-apoptotic protein which can be expressed by this expression vector.
The minimal CMV promoter ensures a continuous expression of the protein, but furthermore it can be expressed in an inducible form using the Tetracyclin-controlled transcriptional activation system.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 778
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |