Composite

Part:BBa_K568001:Design

Designed by: Team TU Munich 2011   Group: iGEM11_TU_Munich   (2011-09-01)
Revision as of 11:37, 1 September 2011 by Registry (Talk | contribs)

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Optogenetical AND-Gate red/blue light


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 644
    Illegal XhoI site found at 1786
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 971
    Illegal AgeI site found at 3817
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3820


Design Notes

We used YcgF/E as blue light sensor and Cph8 as red light sensor. We expect that this assembly will be the one with the lowest amount of unspecific gene expression which could occur due to overlapping of the absorption spectrums of the sensory domains.


Source

designed from existing Biobricks

References