Regulatory
Part:BBa_K639002:Design
Designed by: Espen Bang Leistad Group: iGEM11_NTNU_Trondheim (2011-08-26)
rrnB P1 promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The BioBrick that the Berkeley team made is in BBb format. This means that the BioBrick is flanked by a prefix and a suffix that is different from the ones that are normally used. Therefore, this BioBrick was initially not compatible with the other BioBricks of normal standard (BBa). To deal with this problem, the rrnB P1 promoter was amplified using PCR. The original promoter made by the Berkeley iGEM team was used as template DNA. To make the amplified brick compatible with the rest of our construct, the standard prefix and suffix was added to our primers.
Source
BBa_K112118, rrnB P1 promoter BioBrick in BBb format made by the Berkley iGEM team in 2008.