Composite

Part:BBa_K639000

Designed by: Eirik Selsaas   Group: iGEM11_NTNU_Trondheim   (2011-08-26)
Revision as of 07:54, 26 August 2011 by Registry (Talk | contribs)

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LacI-pLac-mCherry with RBS and terminator

This brick will yield red colonies as it is. When a promoter is inserted in front of the brick, the red color will disappear or be weaker, due to production of LacI that repress lac-promoter. One way of using this brick is to insert a promoter that you suspect, or know is repressed by a molecule or other stimulus. When the promoter is inhibited, transcription of LacI will stop and mCherry will be produced. Thus, the stimuli can be directly measured as intensity of red colour or fluorescense.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1165
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None