Coding
crtE-AB

Part:BBa_K349101:Experience

Designed by: David Lloyd   Group: iGEM10_Alberta   (2010-10-20)
Revision as of 19:53, 11 August 2011 by KarinaA (Talk | contribs) (→‎User Reviews)

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This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K349101

Digestion at the BsaI cut sites inside of the prefix and suffix will create an A overhang at the 5' end and a B overhang at the 3' end. These ends are consistent with the BioBytes 2.0 assembly method.

User Reviews

UNIQ2f33110606315d5f-partinfo-00000000-QINU Karina Arnesen- Alberta iGEM 2010 team- I've noticed that the B' end was designed in such a way that the stop codon is incorrectly placed, or non-existent when used with BA parts using the BioBytes v2.0 assembly method. UNIQ2f33110606315d5f-partinfo-00000001-QINU