Coding

Part:BBa_K523010

Designed by: Mun Ching Lee, Sylvia Ispasanie, Allan Crossman   Group: iGEM11_Edinburgh   (2011-08-04)
Revision as of 08:58, 11 August 2011 by Allancrossman (Talk | contribs)

β-glucosidase for BioSandwich

This is a modified version of BBa_K392008 for use with the BioSandwich protocol of Edinburgh 2011.

BioSandwich assembly uses an in-frame BglII site (formed by the last 2 bases of the RFC10 prefix and the first 4 bases at the start of this BioBrick) and an in-frame SpeI site (present in the RFC10 suffix). Because the SpeI site is out of frame, two extra "G" bases have been added at the end. When combined with the T at the start of the RFC10 suffix, they code for a glycine residue and correct the frameshift.

We discovered late on that the first 220 or so bases are not coding; the part needs to be remade.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 863
    Illegal XhoI site found at 1097
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 350
    Illegal AgeI site found at 246
    Illegal AgeI site found at 441
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 223


[edit]
Categories
Parameters
None