Coding

Part:BBa_K523011

Designed by: Sylvia Ispasanie, Mun Ching Lee   Group: iGEM11_Edinburgh   (2011-08-04)
Revision as of 16:03, 4 August 2011 by Registry (Talk | contribs)

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Endoglucanase for BioSandwich

This is a modified version of BBa_K118023 for use with the BioSandwich protocol of Edinburgh 2011.

BioSandwich assembly uses an in-frame BglII site (formed by the last 2 bases of the RFC10 prefix and the first 4 bases at the start of this BioBrick) and an in-frame SpeI site (present in the RFC10 suffix). Because the SpeI site is out of frame, two extra "G" bases have been added at the end. When combined with the T at the start of the RFC10 suffix, they code for a glycine residue and correct the frameshift.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1092
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 141
    Illegal XhoI site found at 503
    Illegal XhoI site found at 752
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 283
    Illegal NgoMIV site found at 1208
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 187


[edit]
Categories
Parameters
None