Coding

Part:BBa_K523009

Designed by: Sylvia Ispasanie, Mun Ching Lee   Group: iGEM11_Edinburgh   (2011-08-04)
Revision as of 15:45, 4 August 2011 by Allancrossman (Talk | contribs)

Exoglucanase for BioSandwich

This is a modified version of BBa_K118022 for use with the BioSandwich protocol of Edinburgh 2011.

BioSandwich assembly uses an in-frame BglII site (formed by the last 2 bases of the RFC10 prefix and the first 4 bases at the start of this BioBrick) and an in-frame SpeI site (present in the RFC10 suffix). Because the SpeI site is out of frame, two bases ("TC") have been added at the end. When combined with the T at the start of the RFC10 suffix, they code for a serine residue and correct the frameshift.

The signal peptide of the exoglucanase (amino acids 1 - 42) has been removed, since this part is intended for fusion to INP or other display proteins.

Usage and Biology

This is an exoglucanase from Cellulomonas fimi. It degrades cellulose by chewing at the end of a cellulose chain to produce cellobiose sugar.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 405
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 38
    Illegal NgoMIV site found at 411
    Illegal NgoMIV site found at 913
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 458
    Illegal SapI.rc site found at 541


[edit]
Categories
Parameters
None