Composite

Part:BBa_K523006

Designed by: Mun Ching Lee, Sylvia Ispasanie, Allan Crossman   Group: iGEM11_Edinburgh   (2011-07-29)
Revision as of 11:28, 29 July 2011 by Allancrossman (Talk | contribs)

Plac + LacZ + malS

E. coli periplasmic alpha-amylase gene malS under control of lac promoter. LacZ is also present for blue/white screening.

This contains the native malS RBS.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1841
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 607
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 977
    Illegal AgeI site found at 2142
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None