Coding
Part:BBa_K523003
Designed by: Mun Ching Lee, Sylvia Ispasanie Group: iGEM11_Edinburgh (2011-07-29)
malS for BioSandwich
This is a version of BBa_K523001 but with no RBS and no stop codon.
There are two extra bases at the end which, when combined with the T at the start of the standard RFC10 suffix, code for a glycine. This part is intended to be used with Edinburgh 2011's "BioSandwich" protocol, in which a BglII site before the coding sequence, and a SpeI site after the coding sequence (i.e. the one in the suffix) are used for assembly.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1204
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 340
Illegal AgeI site found at 1505 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |