Coding
GFP:LacI

Part:BBa_K606020

Designed by: Kvin Yauy   Group: iGEM11_Paris_Bettencourt   (2011-07-27)
Revision as of 14:11, 27 July 2011 by Kyauy (Talk | contribs)

GFP:LacI protein fusion

GFP:LacI protein fusion.
Use to focalise fluorescence in few spots.
It binds on LacO array (BBa_K606021) (containing 10kb of LacO).
We use it to prove the existence of nanotube between B.subtilis as a proof of concept.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 733
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644


[edit]
Categories
Parameters
None