Coding
GFP:LacI

Part:BBa_K606020:Design

Designed by: Kvin Yauy   Group: iGEM11_Paris_Bettencourt   (2011-07-27)
Revision as of 14:01, 27 July 2011 by Kyauy (Talk | contribs) (→‎References)

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GFP:LacI protein fusion


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 733
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644


Design Notes

2 Quick Change Mutagenesis on the basic sequence has been done


Source

Andrew Wright, Dave Lane, pSG20 plasmid.

References

Chromosome and Low Copy Plasmid Segregation in E. coli: Visual Evidence for Distinct Mechanisms, G. Scott Gordon et al, Cell, 1997
Kinetics of plasmid segregation in Escherichia coli, G. Scott Gordon et al, Molecular Microbiology, 2004