Translational_Unit

Part:BBa_K523002

Designed by: Sylvia Ispasanie, Mun Ching Lee   Group: iGEM11_Edinburgh   (2011-07-19)
Revision as of 17:52, 19 July 2011 by Allancrossman (Talk | contribs)

RBS + bglX (E. coli perisplasmic β-glucosidase)

This is the E. coli beta-glucosidase gene bglX. The part contains the native Ribosome Binding Site.

The part was made using the strategy outlined in BBa_K523000, and therefore contains 4 extra bases at the 5' end which generate a BglII restriction site.

The natural coding sequence contains a PstI site. This part will be edited to be compliant with RFC10 if and when site-specific mutagenesis has been carried out.

Usage and Biology

The product protein is believed to be periplasmic. Beta-glucosidase cleaves Β(1→4) bonds, i.e. those found in the disaccharide cellobiose.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1658
    Illegal AgeI site found at 1880
    Illegal AgeI site found at 2069
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None