Part:BBa_M36698
Pyruvate Sensor v2
Similar to BBa_M36708, this provides a way to test for pyruvate through IclR inhibition of PaceBAK (a portion of the aceBAK promoter). IclR binds with pyruvate to form a more stable structure to bind to the inhibitory regulation sites on the aceBAK promoter. By combining the IclR gene and the aceBAK promoter into the same plasmid, the presence of IclR and pyruvate will inhibit the aceBAK promoter and any genes downstream of it. While there will be some residual transcription in the presence of only IclR, the presence of pyruvate increases the stability of binding with the aceBAK promoter region so inhibitory activities are higher. We choose to order to pieces of DNA to test the influence of signal leveling between the frequency of the IclR gene and aceBAK promoter. In this composite, the IclR gene is occurring in an equal frequency as the aceBAK promoter. See the Part Design for more information accounting for the ability of IclR to inhibit the aceBAK promoter.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 679
Illegal BamHI site found at 1196 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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