Coding

Part:BBa_M36706:Design

Designed by: Katie Lund and Wyatt Woodson   Group: Stanford BIOE44 - S11   (2011-04-26)
Revision as of 21:11, 5 May 2011 by Synbio (Talk | contribs) (→‎Design Notes)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Gene encoding IclR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 597
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Since IclR is naturally made in E. coli, we did not need to do any codon optimization, and the start and stop codons were already included.

Source

This coding sequence was obtained from genome.jp The IclR gene is highlighted here in blue: http://www.genome.jp/kegg-bin/cut_sequence_genes.pl?FROM=5092368&TO=5094223&VECTOR=1&ORG=ece&CHR=c

References