Reporter

Part:BBa_J04430:Design

Designed by: Kristen DeCelle   Group: iGEM2005   (2005-06-09)
Revision as of 03:46, 30 December 2010 by Acyang (Talk | contribs) (→‎Design Notes)

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GFP coding device switched on by IPTG


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 870


Design Notes

Primers: Note: EcoRI sites removed. Must use XP. Base pairs in CAPS are the truncated prefix and the suffix. Sequences provided by Gingko BioWorks.

Fwd: GCTTCTAGAGATACATGAAGATGCACTTcaatacgcaaaccgcctctccc

Rv: CTGCAGCGGCCGCTACTAGTAAGGAGCGCTTtataaacgcagaaaggcccacccg

Source

Davidson Synth-Aces

References