Reporter

Part:BBa_J70657:Design

Designed by: Julie Norville   Group: Knight Lab   (2010-12-20)
Revision as of 23:40, 20 December 2010 by Norville (Talk | contribs) (→‎Design Notes)

N-terminal 6 his fusion test for GFP J70610w604 inserts


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 863


Design Notes

Made from J70610 using the following inserts


J70604f fromJ70610 5'- AGG AGA AAT ACT AGA TGC ATC ATC ATC ATC ATC ACA GCA GCG GCC GTA -3'

J70604r fromJ70610

5'- GGC CGC TGC TGT GAT GAT GAT GAT GAT GCA TCT AGT ATT TCT CCT CTT -3'

Source

Made using bioscaffolds

References