Coding

Part:BBa_J70603:Design

Designed by: Julie Norville   Group: Knight Lab   (2010-06-28)
Revision as of 17:39, 19 November 2010 by Acyang (Talk | contribs) (→‎Design Notes)

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VioE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Primer Design Notes:

Forward primer: gtttcttcgaattcgcggccgcttctagatggagaaccgtgagccaccac l:50 P(g/b):g Ho(max#):-104.84 Het(max#):-112.52 Tm:71.1 GC(%): 56

Reverse primer: gtttcttcctgcagcggccgctactagtattattagcgcttggccgcgaaaac l:53 P(g/b):b Ho(max#):-112.52 Het(max#):-112.52 Tm:70.6 GC(%):52.8

Source

Designed

References