Coding

Part:BBa_J70600:Design

Designed by: Julie Norville   Group: Knight Lab   (2010-06-28)
Revision as of 17:38, 19 November 2010 by Acyang (Talk | contribs) (→‎Design Notes)

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VioB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2523
    Illegal AgeI site found at 2719
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Primer Design Notes:

Forward primer: gtttcttcgaattcgcggccgcttctagatgagcattctggatttcccgcgtatc l: 55 P(g/b): g Ho(max#): -114.97 Het(max#): -114.97 Tm: 70.0 GC(%): 50.9

Reverse primer: gtttcttcctgcagcggccgctactagtattattaggcctcgcggctcagtttg l: 54 P(g/b): b Ho(max#): -111.67 Het(max#): -114.97 Tm: 70.7 GC(%): 53.7

Source

designed

References