Part:BBa_K330002

gusA reporter gene

The gusA reporter gene encodes beta-glucuronidase (GUS), an enzyme originated from Escherichia coli.

Originally, the GUS reporter was designed to be used in plant biology to measure the activity of a promoter. The reporter was later adopted for cell screening and promoter measurement in bacterial cells. There are three commonly used methods: histochemical assay, fluorimetrical assay and spectrophotometric assay. The GUS substrates for these assays differ: 5-bromo-4-chloro-3-indolyl glucuronide for histochemical assay, 4-methylumbelliferyl-beta-D-glucuronide for fluorimetrical assay, p-nitrophenyl β-D-glucuronide for spectrophotometric assay.

THE CHARACTERIZATION RESULTS OF BBa_K330002 IS UNDER 'EXPERIENCE' PAGE (https://parts.igem.org/Part:BBa_K330002:Experience).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]