Part:BBa_I712019:Experience

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

User Reviews

UNIQ06c86fce10948c20-partinfo-00000000-QINU

••• theos

The Cambridge team confirmed that this part did emit light from E. coli when placed under an expression vector (:BBa_J13002) with the addition of D-luciferin. Despite the claim that the "part contains a Eukaryotic ribosome binding site and non-standard prefix and suffix" we were able to express it in E. coli after adding a prokaryotic RBS and promoter using standard BioBrick assembly.

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UNIQ06c86fce10948c20-partinfo-00000002-QINU The Tianjin 2010 igem team confirmed that this part works well since we have used this part to construct our parts which separate the luciferase gene. Our sequencing result reveal that the quality of this part is ok. UNIQ06c86fce10948c20-partinfo-00000003-QINU