DNA

Part:BBa_K419014:Experience

Designed by: YANG TUNG CHIN   Group: iGEM10_TzuChiU_Formosa   (2010-10-24)
Revision as of 17:43, 6 November 2010 by Hewyaohua (Talk | contribs) (→‎Applications of BBa_K419014)

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Applications of BBa_K419014

Verification of the Part BBa_K419014 The Lux I gene with ribosome binding site (RBS) DNA was amplified from the part BBa_C0161. The Lux I PCR products (585 bp) were digested with EcoR I and Pst I, then ligated with the EcoR I-Pst I treated pSB1C3. After E. coli transformation and plasmid preparation, the part BBa_K419014 for sensing system in our Neutrient Synthesizer was verified by EcoRI and PstI enzyme digestion in 1% agarose gel electrophoresis. The arrow (red) indicates the Lux I DNA fragment. The sensing system composite was transformed into E. coli for further experiments.

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User Reviews

UNIQ20bdbe1fd9ff8a8a-partinfo-00000000-QINU UNIQ20bdbe1fd9ff8a8a-partinfo-00000001-QINU