Coding
RFP-AB-Bsa

Part:BBa_K349001:Experience

Designed by: David Lloyd   Group: iGEM10_Alberta   (2010-10-20)
Revision as of 07:23, 6 November 2010 by AlinaP (Talk | contribs) (→‎Applications of BBa_K349001)

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This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K349001

Digestion with BsaI around the recognition sites inside of the prefix and suffix will create an RFP cassette with an A overhang at the 5' end and a B overhang at the 3' end. These ends are consistent with the BioBytes 2.0 assembly method. This is a base plasmid, meaning that the RFP cassette could be digested out with BsaI and replaced with a PCR product that has had BsaI recognition sites and the appropriate AB ends incorporated into it. This allows for the production of more novel AB Bytes.

User Reviews

UNIQd60483e7ea81e512-partinfo-00000000-QINU UNIQd60483e7ea81e512-partinfo-00000001-QINU