Tag

Part:BBa_K496003

Designed by: Shunichi MAKINO   Group: iGEM10_HokkaidoU_Japan   (2010-10-17)
Revision as of 14:17, 5 November 2010 by Sprkata (Talk | contribs)


HokkaidoU Japan T3SS.PNG

RBS + SlrP secretion tag, salmonella T3 sec. sys.

Leucine-rich repeat protein (SlrP) One of the secretion signals in Salmonella Type 3 Secretion System(T3SS). In theory can be used to secrete tagged proteins through Type 3 Secretion Apparatus(T3SA). Due to functional limitations of T3SA it's known that it can't transport very stable proteins like zinc fingers. Regardless it was shown to transport such stable proteins as GFP.

See BBa_K496004 for attempts to use this part to inject GFP to eukaryotic cell.

References

  1. Miao EA, Miller SI. 2000. A conserved amino acid sequence directing intracellular type III secretion by Salmonella typhimurium. Proc Natl Acad Sci U S A. Vol.97:7539-7544. [http://www.ncbi.nlm.nih.gov/pubmed/10861017 PubMed]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None