Reporter

Part:BBa_K313003

Designed by: Ryosuke Kamei, Asahito Kaneko, Kosuke Uekusa, Ryo Kariyazono   Group: iGEM10_UT-Tokyo   (2010-10-20)
Revision as of 05:37, 5 November 2010 by Misoramen (Talk | contribs)

terminator(reverse direction) and gfp (reverse direction)

Reverse reporter(gfp and double terminator in the reverse dircion).

Please see [http://2010.igem.org/Team:UT-Tokyo/Sudoku_assay_LocSq Location sequence] assay page.

Figure

  • The result of gfp expression assay

Transcription of gfp reverse from the pBAD reverse promoter was induced by 10x10^(-2)M arabinose when OD_(600) reached 0.1 to 0.2.

Plasmid was transformed into JM109.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 209


[edit]
Categories
Parameters
None