Composite

Part:BBa_K329055

Designed by: Xavier Duportet   Group: iGEM10_Paris_Liliane_Bettencourt   (2010-10-25)
Revision as of 02:28, 5 November 2010 by Adecrulle (Talk | contribs)

[KanR + LacZ] in between MUT_MUT[Lambda] Tn916 Arms

This part is composed of a Kanamycine resistance and LacZ cassette between two mutated arms (left and right) of the transposon Tn916. When the both enzyme of the transposon,Int and Xis tn916 are expressed, the both arms link together forming a circular DNA sequence excised from the hosting DNA (plasmid or chromosome).


Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 363
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 382
    Illegal NheI site found at 405
    Illegal SpeI site found at 363
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 363
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 363
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None