Part:BBa_K321003

intracellular chain of CD3 zeta

Encodes the intracellular signaling domain of the CD3 zeta molecule of the T-Cell Receptor complex. The sequence encodes the three ITAMs (Immunoreceptor tyrosine-based activation motif) that can activate immune cells.

Usage and Biology

The CD3 zeta molecule activates immune T cells upon ligand binding to the T-Cell Receptor complex. The immunoreceptor tyrosine-based activation (ITAM) motifs in the intracellular domain of CD3 zeta recruits kinases that activate downstream molecules involved in cell signaling. This intracellular domain has been shown to function in a modular manner. It can be fused to different extracellular ligand binding domains to create functional synthetic receptors.

Function

This part was shown to activate killer cells by the UCSF 2010 team. We successfully used this part in our proof of concept logic gate design for greater precision of killing towards cancer cells. The figure below shows that the part performs its activating function as expected: it activates killer cells in the presence of the intended ligand A that activates the synthetic receptor bearing this part (left chart, green bar). For more information please see our wiki.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 274