Part:BBa_K321004

intracellular chain of KIR3DL1

Encodes the intracellular signaling chain of the KIR3DL1 immune receptor. Inhibits immune cell activation via the ITIM (immunoreceptor tyrosine-based inhibition motif) motif.

Usage and Biology

In immune Natural Killer cells, Killer cell immunoglobulin-like receptors (KIRs) inhibit cell mediated cytotoxicity upon ligand binding. In their intracellular domains, these receptors possess immune tyrosine-based inhibitory (ITIM) motifs that when phosphorylated recruit phosphotases that decrease the activation molecules involved in immune cell signaling.

Function

This part was shown to inhibit the activation of killer cells by the UCSF 2010 team. We used this part in our ANDN gate to increase the precision of killing toward cancer cells. The figure below shows that this part performs its inhibitory function as expected: it inhibits killer cell activation in the presence of antigen B, which is recognized by the extracellular domain of the synthetic receptor (red and striped bars). For more information please see our wiki.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]