Part:BBa_K321004

intracellular chain of KIR3DL1

Encodes the intracellular signaling chain of the KIR3DL1 immune receptor. Inhibits immune cell activation via the ITIM (immunoreceptor tyrosine-based inhibition motif) motif.

Usage and Biology

In wild-type NK cells, Killer cell immunoglobulin-like receptors (KIRs) with long cytoplasmic domains inhibit cell mediated cytotoxicity upon ligand binding. They possess a immune tyrosine-based inhibitory motif (ITIM) that when phosphorylated recruits phosphotases like SHP-1 that decrease the activation molecules involved in cell signaling.

Function

This part was shown to inhibit the activation of killer cells by the UCSF 2010 team. We used this part in our ANDN gate to increase the precision of killing toward cancer cells. The figure below shows that this part performs its inhibitory function as expected: it inhibits killer cell activation in the presence of antigen B, which is recognized by the extracellular domain of the synthetic receptor. For more information please see our wiki.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]