Reporter

Part:BBa_K313000

Designed by: Kohaku So, Mei Kobayashi, Haruka Agatsuma, Ryosuke Kamei, Ryo Kariyazono   Group: iGEM10_UT-Tokyo   (2010-10-02)
Revision as of 13:13, 2 November 2010 by Kohaku (Talk | contribs)

gfp generator with T7 promoter

gfp generator with T7 promoter

Usage and Biology

T7 RNA polymerase induces the expression of gfp from this part.

Please see [http://2010.igem.org/Team:UT-Tokyo/Sudoku_assay_LeakSw Terminator leak] assay page.

Verification Experiment

Plasmid containing this part was transformed to Rosetta (DE3) plysS. When the OD reached 0.6, we added IPTG to a final concentration of 0.1 mM Fluorescence was measured and the results are shown below:

Graph 5.png


Sequence and Features



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 719


[edit]
Categories
Parameters
None