Part:BBa_K415508

phEF1a L4R1 MammoBlock Entry Vector

L4R1 Mammoblock Promoter Vector containing human elongation factor 1a (hEF1a) promoter. hEF1a is a low level constitutive promoter.

Characterization

Figure 1. Fluorescence of HEK293FT cells 7 days post infection with hEF1a_EGFP construct.

HEK293 FT cells were infected with replication-deficient HIV particles carrying the hEF1a_EGFP construct. Fluorescent micrographs were taken 7 days after the procedure and show low level constitutive expression from EGFP under the control of the hEF1a promoter.

                                                                                                                                                                                                                                     

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 6
  Illegal EcoRI site found at 1235
  Illegal PstI site found at 361
  Illegal PstI site found at 866
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 6
  Illegal EcoRI site found at 1235
  Illegal PstI site found at 361
  Illegal PstI site found at 866
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 6
  Illegal EcoRI site found at 1235
  Illegal BglII site found at 615
  Illegal XhoI site found at 1014
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 6
  Illegal EcoRI site found at 1235
  Illegal PstI site found at 361
  Illegal PstI site found at 866
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 6
  Illegal EcoRI site found at 1235
  Illegal PstI site found at 361
  Illegal PstI site found at 866
  Illegal NgoMIV site found at 749
  Illegal AgeI site found at 127
  
• 1000
  COMPATIBLE WITH RFC[1000]