Translational_Unit

Part:BBa_K371003:Design

Designed by: Yang Zhang   Group: iGEM10_USTC   (2010-08-27)
Revision as of 08:38, 31 October 2010 by Zocean (Talk | contribs) (→‎Source)

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pduAB+pduJK


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1686
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There are some silent mutation in pduAB, but do not disturb the function of protein. PduJK has some error mutations at the end of coding sequence of pduK. We are still doing experiment do confirm whether this part can work normally.


Source

pduAB come from Citrobacter Freundii genome(NBRC NO.12681) pduJK come from Citrobacter Freundii genome(NBRC NO.12681)

References