Coding

Part:BBa_K339002

Designed by: Christopher Tang   Group: iGEM10_Calgary   (2010-10-23)
Revision as of 02:01, 30 October 2010 by Emily Hicks (Talk | contribs)

malEΔSS

This is a form of the maltose binding protien (malE) native to E. Coli, tht has the signal sequence removed.


Usage and Biology

With the signal sequence deleted, this protein is not localized to the periplasm, but rather stays in the cytoplasm. Despite this, it is still able to fold well in the cytoplasm. This protein can therefore be used as a fusion partner for hard to express proteins that stay in the cytoplasm of the cell. This part can also be used to characterize cytoplasmic misfolding promoters.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 360
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 58


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Categories
Parameters
None