Coding

Part:BBa_K339001

Designed by: Emily Hicks   Group: iGEM10_Calgary   (2010-10-23)
Revision as of 23:24, 29 October 2010 by Emily Hicks (Talk | contribs)

Mutant Maltose Binding Protein (malE31)

This mutant form of maltose binding protein contains a two amino acid mutation at amino acid positions 33 and 34 causing the protein to have difficulty folding properly.

Usage and Biology

This protein can be used to test and characterize various stress promoters.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 435
    Illegal BamHI site found at 171
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 133


[edit]
Categories
Parameters
None