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• Part:BBa K4762005
  CAT expressed in E. coli can break down hydrogen peroxide (H2O2) into water and <b>Figure 1. </b>The recombinant plasmid pET-28a(+)-CAT was transformed into DH5α.
  4 KB (559 words) - 06:25, 12 October 2023
• Part:BBa K1470000
  ...dependent of Na⁺. Additionally it was shown that the absence of CAT-1 leads to non-viable mice pubs [1][2].</p> ...murine leukeamia virus is only able to enter the cell, when it detects the CAT-1 sugar-bound moieties[3].</p><br>
  6 KB (987 words) - 21:13, 2 November 2014
• Part:BBa K3523006
  ...nd efficiently. To achieve it, we selected a classical enzymes -- catalase(CAT), which are capable of effectively degrading ROS, for overexpression and pu ...ator to end transcription. At the same time, insert a His protein tag into CAT protein for purification of protein on the nickel column. This part can b
  12 KB (1,718 words) - 03:49, 22 October 2021
• Part:BBa K4762014
  <b>Figure 3. </b> The recombinant plasmid pET-28a(+)-T5-CAT-SOD was transformed into EcN. <b>Figure 4. </b>The recombinant plasmid pET-28a(+)-T5-CAT-SOD-Elafin was transformed into DH5α.
  5 KB (655 words) - 15:46, 12 October 2023
• Part:BBa K2936018
  ...of HCHO in the environment reach a certain level. The catalase encoded by CAT gene could be reacted with TMB to make the culture blue. ...of HCHO in the environment reach a certain level. The catalase encoded by CAT gene could be reacted with TMB to make the culture blue.
  6 KB (933 words) - 03:58, 22 October 2019
• Part:BBa J73101:Design
  ATG AAC CAC CAA ATT CAT ATC CAC GAC AGC GAT ATT GCT TTC CCG TGT AAA TTC GAA GCG GGT CAA TAT CTT TTG ATT CAT TTA GAC GAC GGT GAG
  1 KB (355 words) - 17:17, 14 April 2023
• Part:BBa K4730000:Design
  ...CA GCT GAC GGT CAA GCG GCG GAT TTT GCT CGT TTG TTC ATT ATT CCC GGG ATG AAC CAT TGC CAA GGT GGT CCC GCC ACA GAT GAC GTT GAT TTA TTG ACT CCG CTT ATG GCG TGG
  3 KB (624 words) - 07:03, 6 October 2023
• Part:BBa K4645003
  ...oteins were connected with a flexible linker to enable them to colonize on cat tooth and form a stable biofilm, creating conditions for the realization of ...in the same tube of cat saliva at 37℃ and 200 rpm for 14 hours. Then The cat teeth incubated with saliva were removed, and each tooth fragment was place
  12 KB (1,858 words) - 15:14, 12 October 2023
• Part:BBa K4762017
  Superoxide dismutase (SOD) and catalase (CAT) are two kinds of antioxidant enzymes that are ubiquitous in various organi <b>Figure 1. </b>The function diagram of CAT-SOD.
  3 KB (311 words) - 08:19, 12 October 2023
• Part:BBa K808025
  ===== K_cat calculation ===== ...through dividing V_max by the enzyme concentration of 5nM. K_cat counts 0,293395 1/mol*s.
  13 KB (2,019 words) - 04:22, 25 August 2020
• Part:BBa K3343000
  ...>BBa_K1614007</a>) and report the molar maximum TMB conversion rate (k_cat) and substrate affinity (K_m) of this part.</p></html> ...ion rate and TMB affinity of EAD2+3’A, respectively. The values of k_cat and K_m were calculated using multiple regression analysis
  9 KB (1,325 words) - 17:09, 27 October 2020
• Part:BBa K3791021
  ...e forward rate constant, K_r the reverse rate constant and K_cat the catalytic rate constant. It is also important to point out the di By defining <b>τ = K_M/K_catE₀</b> and solving analytically the differential equation f
  23 KB (3,520 words) - 00:10, 20 October 2021
• Part:BBa K4339000
  <h4>k_cat</h4> ...nce for the enzyme which binds it (alanine). This yielded a value of k_cat = 0.9953 s^-1.
  7 KB (1,048 words) - 08:31, 12 October 2022
• Part:BBa K4868001
  For the PCR, the Ampliqon 2x Taq red master mix (Cat. No.: A190303) was used and the following PCR program was run: ...d subsequent cell lysis and loading onto a Tris-Glycine gel (Thermo Fisher Cat. No. XP00165BOX).
  22 KB (3,444 words) - 15:56, 12 October 2023
• Part:BBa K4645000
  ...gate the blocking effect of our scFvs (single-chain fragment variables) on cat allergies. Of all known cat allergens, feline domestic allergen 1 (Fel d 1) is the most well-studied. I
  6 KB (994 words) - 14:05, 12 October 2023
• Part:BBa K863020
  ...oomassie staining (Fig. 1). We observed expression of the positive control CAT protein (~32 kDa), but no evidence of tthl or bhal expression (Fig 2.) Fig 3. Absorbance values of laccase proteins (Trametes = Positive control; CAT = Negatice control)
  4 KB (642 words) - 06:42, 11 October 2022
• Part:BBa K2116053
  <h1>Characterisation of the CAT-UPRT fusion protein</h1> ...esponse of the dual selector plasmid containing a CAT-UPRT fusion protein (CAT: chloramphenicol acetyltransferase, UPRT: uracil phosphoribosyltransferase)
  2 KB (255 words) - 15:13, 28 October 2016
• Part:BBa K2728001
  (1) Culture E. coli harboring pFrmR - Cat gene in LB medium with different concentrations of formaldehyde (0 mg/ml, 1 ...g pet-28b-cat plasmid as the control group, and E. coil with pet-28b-pfrmr-cat as the experimental group.
  18 KB (2,616 words) - 09:17, 9 October 2023
• Part:BBa K2936014
  (1) Pick one colony of E. coli harboring Cat genethe into 5 ml LB medium and cultivate it overnight.
  4 KB (518 words) - 03:50, 22 October 2019
• Part:BBa K2926076
  ...ia&nbsp;coli</i>, that mediates resistance to chloramphenicol. (Shaw 1983) CAT covalently attaches an acetyl group from acetyl-CoA to chloramphenicol, ren It is encoded by the <i>cat</i> gene which is commonly used by the iGEM community as part <a href=”ht
  58 KB (7,552 words) - 03:38, 22 October 2019