Part:BBa_J73101:Design
TphA1 reductase
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 279
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 997
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
optimized for E.coli
Source
ATG AAC CAC CAA ATT CAT ATC CAC GAC AGC GAT ATT GCT TTC CCG TGT GCT CCT GGT CAA AGC GTG TTA GAT GCC GCC TTA CAG GCT GGA ATC GAG CTG CCC TAT TCG TGT CGT AAA GGA TCG TGT GGG AAC TGT GCG TCT ACG TTA TTA GAC GGG AAC ATC GCA TCT TTC AAC GGT ATG GCT GTC CGT AAC GAA TTA TGT GCG TCG GAG CAG GTT CTG CTT TGT GGT TGC ACC GCC GCG AGT GAC ATC CGC ATC CAC CCT AGT AGC TTC CGC CGC CTG GAT CCA GAG GCG CGT AAG CGT TTT ACT GCG AAA GTT TAT TCC AAT ACC CTG GCA GCA CCC GAT GTT AGC CTT CTG CGC TTA CGC TTA CCC GTC GGA AAG CGC GCA AAA TTC GAA GCG GGT CAA TAT CTT TTG ATT CAT TTA GAC GAC GGT GAG AGC CGT TCG TAT AGC ATG GCT AAT CCA CCT CAC GAG TCT GAC GGG ATT ACC TTA CAT GTG CGT CAT GTG CCG GGC GGT CGC TTT TCC ACA ATC GTG CAG CAA TTA AAG TCT GGC GAT ACT CTT GAC ATC GAA CTG CCA TTT GGC TCA ATC GCC TTA AAG CCT GAT GAC GCC CGC CCG TTG ATT TGT GTA GCT GGA GGT ACT GGG TTT GCG CCC ATT AAA AGT GTT CTG GAC GAT CTT GCC AAA CGC AAG GTT CAG CGC GAC ATC ACT TTG ATC TGG GGC GCA CGT AAC CCA TCT GGT CTT TAC CTG CCC TCA GCT ATT GAT AAA TGG CGT AAA GTG TGG CCA CAG TTC CGT TAC ATT GCC GCT ATC ACC GAC TTA GGC GAT ATG CCC GCT GAC GCT CAC GCG GGT CGC GTC GAC GAT GCC CTG CGT ACA CAC TTT GGT AAC TTA CAC GAT CAC GTA GTT CAT TGT TGC GGC AGC CCC GCA CTG GTA CAG AGC GTT CGC ACG GCG GCG TCC GAC ATG GGC CTG TTA GCG CAG GAT TTT CAT GCT GAC GTA TTC GCG ACT GGT CCA ACC GGT CAT CAC