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  • ...characteristics of an experiment, such as sensitivity, leakage, etc. DeGFP synthesis rate differs for the same element in different chassis. <br> ...2">(BBa_K2205002)</a> registered by iGEM17_Newcastle in the Parts, but the synthesis rate of deGFP in the Cell-Free system was lower than that in a cell-based s
    9 KB (1,462 words) - 18:13, 21 October 2021
  • ...using the enzyme-catalyzed synthesis route, the yield of enzyme-catalyzed synthesis of 2'-FL is not high due to the expensive substrate guanosine 5’-diphosph ...gmd, wcaG and Wbgl, are encoding the important enzymes involving the 2'-FL synthesis. Amongst these enzymes, gmd and wcaG are converted the abundant intracellul
    16 KB (2,326 words) - 10:22, 27 September 2022
  • ...cloning of a PCR amplified backbone and an insert obtained by solid phase synthesis. Primers for pUC19 backbone amplification were analysed on a temperature gr ...se synthesis derived cassette. Both cassettes derived from the solid-phase synthesis did not yield any visible dsRNA, while our cassette produced a dsRNA at a s
    8 KB (1,375 words) - 15:02, 12 October 2022
  • ...hesis method to synthesize zinc responsive promoter (pzntR) and riboflavin synthesis gene (ribB). ...lavin synthesis gene (ribB) by direct DNA synthesis method. The riboflavin synthesis gene for riboflavin expression promotes electron transfer from the cell to
    5 KB (683 words) - 08:31, 12 October 2022
  • ...a greater yield of AdoCbl, the naturally occurring gene <i>bluB</i> of the synthesis pathway of <i>sinorhizobium meliloti</i> 2011 [3]. ...ogen source despite ethanolamine. Since nitrogen is crucial for amino acid synthesis and ultimately cell survival, <i>E. coli</i> is unable to grow in such a me
    35 KB (5,319 words) - 14:18, 12 October 2023
  • ...growth and replication of ''E. coli'', the processes of DNA design and DNA synthesis cannot be easily decoupled.
    2 KB (304 words) - 17:17, 22 March 2008
  • ...artinfo>pSB1A3</partinfo>). The results are shown here as the relative RFP synthesis rate per cell. |[[Image:PV_hc_r001110LB.png|500px|thumb|Relative RFP synthesis rate per cell in <partinfo>pSB1A2</partinfo>-<partinfo>BBa_J107010</partinf
    22 KB (3,531 words) - 15:09, 11 October 2022
  • ...hesis method to synthesize zinc responsive promoter (pzntR) and riboflavin synthesis gene (ribB). ...lavin synthesis gene (ribB) by direct DNA synthesis method. The riboflavin synthesis gene for riboflavin expression promotes electron transfer from the cell to
    5 KB (715 words) - 08:29, 12 October 2022
  • ...artinfo>pSB1A3</partinfo>). The results are shown here as the relative RFP synthesis rate per cell. |[[Image:PV_hc_r001110LB.png|500px|thumb|Relative RFP synthesis rate per cell in <partinfo>pSB1A2</partinfo>-<partinfo>BBa_J107010</partinf
    9 KB (1,400 words) - 11:20, 30 October 2010
  • [[Image:metabolism.png|thumb|right|200px|Fig.1 synthesis mechanism of P(3HB)]] The pathway and regulation of Poly[(R)-3-hydroxybutyrate] ,P(3HB) synthesis in <I>Ralstonia eutropha</I> H16 is shown in Fig1. Pyruvic acid is metaboli
    8 KB (1,279 words) - 05:49, 13 December 2019
  • ==== Origin: Saccharomyces cerevisiae, Saccharomycopsis fibuligera, synthesis ==== ==== Origin: Saccharomycopsis fibuligera, synthesis ====
    15 KB (2,134 words) - 03:55, 22 October 2021
  • The riboflavin synthesis gene cluster (''rib''-gene-cluster) consists of four different genes that f ...ielefeld 2013 Rib cluster2.png|500px|thumb|left|'''Figure 4:''' Riboflavin synthesis gene cluster, cloned from ''Shewanella oneidensis'' MR-1, 3697 bp; with non
    27 KB (3,978 words) - 04:33, 17 December 2021
  • ===Synthesis of SerA Gene=== ===Plasmid Synthesis===
    5 KB (714 words) - 00:55, 17 October 2016
  • ...eraniol synthesis in E. coli, so at the beginning, we expressed a geraniol synthesis operon (https://parts.igem.org/Parts:BBa_K2753016) containing an <em>Abies .... Thus we decided to co-express an MVA pathway in addition to the geraniol synthesis operon. The pathway can either be added by cotransformation of a plasmid co
    10 KB (1,547 words) - 17:46, 25 October 2020
  • ...eraniol synthesis in E. coli, so at the beginning, we expressed a geraniol synthesis operon (https://parts.igem.org/Parts:BBa_K2753016) containing an <em>Abies .... Thus we decided to co-express an MVA pathway in addition to the geraniol synthesis operon. The pathway can either be added by cotransformation of a plasmid co
    8 KB (1,262 words) - 07:30, 17 October 2018
  • ...gy that not only circumvents the use of toxic reagents for indigo chemical synthesis but also removes the need for a reducing agent for dye solubilization. .... The hidden pathway for production is encoded by the VioABCDE operon. Bio-synthesis starts from L-tryptophan, converted into protodeoxyviolaceinic acid by VioA
    25 KB (3,706 words) - 18:25, 21 October 2021
  • ...ge, 1981). When sugars are used as the carbon source, cytosolic acetyl-CoA synthesis in S. cerevisiae occurs via the concerted action of pyruvate decarboxylase ...ynthesis corresponds to 1 mol of glucose that needs to be respired for the synthesis of 1 mol of product.
    87 KB (12,171 words) - 05:10, 21 October 2021
  • In E. coli, the presence of glucose inhibits the synthesis of many catabolic enzymes, a phenomenon called "glucose effect" or "glucose ...strain contained pMTL83151-J23100-butyrate plasmids and expressed butyrate synthesis gene cluster; placY represents that the host strain contained p15A-J23200-l
    6 KB (839 words) - 14:01, 27 October 2020
  • ...ex responsible for the synthesis positive-sense viral RNAs, results in the synthesis of both the genomic and subgenomic RNAs, of which the subgenomic RNA is pro ...olutely conserved N-terminal tyrosine interacts with nsP1 for minus-strand synthesis. Stollar and colleagues have determined several determinants of promoter bi
    5 KB (714 words) - 21:23, 26 October 2020
  • ...l had lots of drawbacks that it can cause pollution and the substrates for synthesis were harmful to people's health.<br> ...ncluding FMO and sty gene group were discovered useful for bacteria indigo synthesis (Choi et al., 2003, Han, Bang, Lim and Kim, 2010). To achieve our goal of p
    4 KB (564 words) - 10:13, 27 October 2020

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