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  • ...y designed by Shin and Noireaux (2010) for expression in cell-free protein synthesis systems and is more efficiently translated than other variants. ...characteristics of an experiment, such as sensitivity, leakage, etc. DeGFP synthesis rate differs for the same element in different chassis. <br>
    6 KB (892 words) - 14:29, 21 October 2021
  • ...-Proinsulin, both cell lines showed significantly higher rates of glycogen synthesis than basal levels (Figure 3 and 4), and showed some glucose oxidation activ ...eated with Ecotin-Proinsulin showed significantly higher rates of glycogen synthesis than those at basal levels, and a slight increase in glucose oxidation was
    7 KB (1,008 words) - 01:55, 1 November 2017
  • ...-Proinsulin, both cell lines showed significantly higher rates of glycogen synthesis than basal levels (Figure 3 and 4), and also showed significantly greater g ...with Cytoplasmic-Proinsulin showed significantly higher rates of glycogen synthesis and glucose oxidation than basal levels. Significance calculated using an u
    6 KB (804 words) - 01:56, 1 November 2017
  • ...eraniol synthesis in E. coli, so at the beginning, we expressed a geraniol synthesis operon (https://parts.igem.org/Parts:BBa_K2753016) containing an <em>Abies .... Thus we decided to co-express an MVA pathway in addition to the geraniol synthesis operon. The pathway can either be added by cotransformation of a plasmid co
    11 KB (1,720 words) - 08:21, 27 October 2020
  • .... We validated the function of KPD+CFPPK by performing intracellular polyP synthesis using phosphite as the substrate. ...P, +3 valance) as the solo phosphorus source was adopted to evaluate polyP synthesis.
    8 KB (1,161 words) - 13:32, 11 October 2022
  • Polyphosphate kinase (PPK) catalyzes the synthesis of polyphosphate (polyP). PolyP is a polyanionic biopolymer and generally s ...K. We validated the effectiveness of PPK-M on enhanced intracellular polyP synthesis by comparison with PPK.
    5 KB (695 words) - 12:18, 27 September 2022
  • ...ex responsible for the synthesis positive-sense viral RNAs, results in the synthesis of both the genomic and subgenomic RNAs, of which the subgenomic RNA is pro ...the identified interactions of nsP3 with host factors influence viral RNA synthesis or whether they are indicative of another nsP3 function that regulates the
    7 KB (1,096 words) - 08:37, 21 October 2021
  • ...rates to produce acyl CoA thiolipids for subsequent reactions. Through the synthesis of these phenylpropane derivatives CoA lipids (e.g. p-gumaroyl CoA, feruloy ...". It exists widely in nature. ACC is a rate-limiting enzyme for ab initio synthesis of fatty acids, which catalyzes acetyl-CoA to malonyl-CoA, which eventually
    8 KB (1,337 words) - 13:38, 21 October 2021
  • ...rates to produce acyl CoA thiolipids for subsequent reactions. Through the synthesis of these phenylpropane derivatives CoA lipids (e.g. p-gumaroyl CoA, feruloy ...". It exists widely in nature. ACC is a rate-limiting enzyme for ab initio synthesis of fatty acids, which catalyzes acetyl-CoA to malonyl-CoA, which eventually
    8 KB (1,333 words) - 13:38, 21 October 2021
  • ...rates to produce acyl CoA thiolipids for subsequent reactions. Through the synthesis of these phenylpropane derivatives CoA lipids (e.g. p-gumaroyl CoA, feruloy ...". It exists widely in nature. ACC is a rate-limiting enzyme for ab initio synthesis of fatty acids, which catalyzes acetyl-CoA to malonyl-CoA, which eventually
    8 KB (1,329 words) - 13:41, 21 October 2021
  • ...characteristics of an experiment, such as sensitivity, leakage, etc. DeGFP synthesis rate differs for the same element in different chassis. <br> ...2">(BBa_K2205002)</a> registered by iGEM17_Newcastle in the Parts, but the synthesis rate of deGFP in the Cell-Free system was lower than that in a cell-based s
    9 KB (1,462 words) - 18:13, 21 October 2021
  • ...using the enzyme-catalyzed synthesis route, the yield of enzyme-catalyzed synthesis of 2'-FL is not high due to the expensive substrate guanosine 5’-diphosph ...gmd, wcaG and Wbgl, are encoding the important enzymes involving the 2'-FL synthesis. Amongst these enzymes, gmd and wcaG are converted the abundant intracellul
    16 KB (2,326 words) - 10:22, 27 September 2022
  • ...cloning of a PCR amplified backbone and an insert obtained by solid phase synthesis. Primers for pUC19 backbone amplification were analysed on a temperature gr ...se synthesis derived cassette. Both cassettes derived from the solid-phase synthesis did not yield any visible dsRNA, while our cassette produced a dsRNA at a s
    8 KB (1,375 words) - 15:02, 12 October 2022
  • ...hesis method to synthesize zinc responsive promoter (pzntR) and riboflavin synthesis gene (ribB). ...lavin synthesis gene (ribB) by direct DNA synthesis method. The riboflavin synthesis gene for riboflavin expression promotes electron transfer from the cell to
    5 KB (683 words) - 08:31, 12 October 2022
  • ...a greater yield of AdoCbl, the naturally occurring gene <i>bluB</i> of the synthesis pathway of <i>sinorhizobium meliloti</i> 2011 [3]. ...ogen source despite ethanolamine. Since nitrogen is crucial for amino acid synthesis and ultimately cell survival, <i>E. coli</i> is unable to grow in such a me
    35 KB (5,319 words) - 14:18, 12 October 2023
  • ...growth and replication of ''E. coli'', the processes of DNA design and DNA synthesis cannot be easily decoupled.
    2 KB (304 words) - 17:17, 22 March 2008
  • ...artinfo>pSB1A3</partinfo>). The results are shown here as the relative RFP synthesis rate per cell. |[[Image:PV_hc_r001110LB.png|500px|thumb|Relative RFP synthesis rate per cell in <partinfo>pSB1A2</partinfo>-<partinfo>BBa_J107010</partinf
    22 KB (3,531 words) - 15:09, 11 October 2022
  • ...hesis method to synthesize zinc responsive promoter (pzntR) and riboflavin synthesis gene (ribB). ...lavin synthesis gene (ribB) by direct DNA synthesis method. The riboflavin synthesis gene for riboflavin expression promotes electron transfer from the cell to
    5 KB (715 words) - 08:29, 12 October 2022
  • ...artinfo>pSB1A3</partinfo>). The results are shown here as the relative RFP synthesis rate per cell. |[[Image:PV_hc_r001110LB.png|500px|thumb|Relative RFP synthesis rate per cell in <partinfo>pSB1A2</partinfo>-<partinfo>BBa_J107010</partinf
    9 KB (1,400 words) - 11:20, 30 October 2010
  • [[Image:metabolism.png|thumb|right|200px|Fig.1 synthesis mechanism of P(3HB)]] The pathway and regulation of Poly[(R)-3-hydroxybutyrate] ,P(3HB) synthesis in <I>Ralstonia eutropha</I> H16 is shown in Fig1. Pyruvic acid is metaboli
    8 KB (1,279 words) - 05:49, 13 December 2019

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