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• Part:BBa K4085015
  GDP-L-fucose is a key precursor for the synthesis of 2-FL, and it can be synthesized through two pathways. One is the de novo ...using the enzyme-catalyzed synthesis route, the yield of enzyme-catalyzed synthesis of 2'-FL is not high due to the expensive substrate guanosine 5’-diphosph
  16 KB (2,401 words) - 14:44, 12 October 2022
• Part:BBa K4885006
  ...yl-CoA dehydrogenase are rate-limiting enzymes in the butyrate and butanol synthesis pathway. ...engineer C. tyrobutyricum to overexpressed several enzymes to enhance the synthesis pathway of butyrate and butanol. BBa_K4885002 were constructed to enhance t
  6 KB (828 words) - 13:29, 12 October 2023
• Plasmid backbones/Synthesis
  ==Plasmids used in DNA synthesis of BioBrick parts== ...t DNA synthesis. Here is a list of plasmid backbones used by assorted DNA synthesis companies.
  981 B (133 words) - 16:41, 22 December 2008
• Part:BBa K528004
  ===The mechanism of A/U-rich 5'-UTR to promote protein synthesis=== Fig.1. The mechanism of A/U-rich 5'-UTR to promote protein synthesis.<BR>
  4 KB (565 words) - 01:26, 22 October 2019
• Part:BBa K554005
  Origin: synthesis ...We thus decided to create a completely new biobrick, responsible for Il-12 synthesis. The IL-12 gene is used by [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil U
  8 KB (1,266 words) - 07:53, 6 October 2022
• Part:BBa K759004
  Bacterial cellulose-PHB composite was achieved by introducing PHB synthesis operon into <i> K. xylinus</i>. This way, bacteria are able to simultaneous ...719017">BBa_K4719017</a>, containing <i>phaA</i>, is a polyhydroxybutyrate synthesis operon (<i>phaC, phaA, phaB</i>), producing PHB along with bacterial cellul
  4 KB (591 words) - 14:50, 12 October 2023
• Part:BBa K2110105
  ...ethod in molecular biology. Production of proteins using cell-free protein synthesis usually takes a few hours, in contrast to production of proteins in cells, ...reeening of enzymes activities. In the post-genomic era, cell-free protein synthesis has rapidly become the preferred approach for high-throughput functional an
  6 KB (859 words) - 12:03, 20 October 2016
• Part:BBa K1973012
  ...ve the implementation of a system that allows us to control externally the synthesis and turn-down of c-diGMP, we had cloned both genes pleD* and yhjH separatel ...of PleD* increases the intracellular concentration of c-diGMP, whereas the synthesis of YhjH decreases the intracellular concentration of this second messenger.
  10 KB (1,550 words) - 22:52, 19 October 2016
• Part:BBa K1973023
  ...ve the implementation of a system that allows us to control externally the synthesis and turn-down of c-diGMP, we had cloned both genes pleD* and yhjH separatel ...of PleD* increases the intracellular concentration of c-diGMP, whereas the synthesis of YhjH decreases the intracellular concentration of this second messenger.
  10 KB (1,526 words) - 22:50, 19 October 2016
• Part:BBa K274001
  ...s: League of Enhanced MicroGladiators." One of our methods of nanoparticle synthesis and attachment to cells is titled the recombinant method. Through the use o With this data, we intended to perform nanoparticle synthesis with L-tyrosine and MelA-transformed <i>E. coli</i> cells, and have these c
  11 KB (1,605 words) - 15:27, 12 October 2022
• Part:BBa K2205002
  ...y designed by Shin and Noireaux (2010) for expression in cell-free protein synthesis systems and is more efficiently translated than other variants. ...characteristics of an experiment, such as sensitivity, leakage, etc. DeGFP synthesis rate differs for the same element in different chassis. <br>
  6 KB (892 words) - 14:29, 21 October 2021
• Part:BBa K2417000
  ...-Proinsulin, both cell lines showed significantly higher rates of glycogen synthesis than basal levels (Figure 3 and 4), and showed some glucose oxidation activ ...eated with Ecotin-Proinsulin showed significantly higher rates of glycogen synthesis than those at basal levels, and a slight increase in glucose oxidation was
  7 KB (1,008 words) - 01:55, 1 November 2017
• Part:BBa K2417001
  ...-Proinsulin, both cell lines showed significantly higher rates of glycogen synthesis than basal levels (Figure 3 and 4), and also showed significantly greater g ...with Cytoplasmic-Proinsulin showed significantly higher rates of glycogen synthesis and glucose oxidation than basal levels. Significance calculated using an u
  6 KB (804 words) - 01:56, 1 November 2017
• Part:BBa K2753015
  ...eraniol synthesis in E. coli, so at the beginning, we expressed a geraniol synthesis operon (https://parts.igem.org/Parts:BBa_K2753016) containing an <em>Abies .... Thus we decided to co-express an MVA pathway in addition to the geraniol synthesis operon. The pathway can either be added by cotransformation of a plasmid co
  11 KB (1,720 words) - 08:21, 27 October 2020
• Part:BBa K3022002
  .... We validated the function of KPD+CFPPK by performing intracellular polyP synthesis using phosphite as the substrate. ...P, +3 valance) as the solo phosphorus source was adopted to evaluate polyP synthesis.
  8 KB (1,161 words) - 13:32, 11 October 2022
• Part:BBa K1807009
  Polyphosphate kinase (PPK) catalyzes the synthesis of polyphosphate (polyP). PolyP is a polyanionic biopolymer and generally s ...K. We validated the effectiveness of PPK-M on enhanced intracellular polyP synthesis by comparison with PPK.
  5 KB (695 words) - 12:18, 27 September 2022
• Part:BBa K3504002
  ...ex responsible for the synthesis positive-sense viral RNAs, results in the synthesis of both the genomic and subgenomic RNAs, of which the subgenomic RNA is pro ...the identified interactions of nsP3 with host factors influence viral RNA synthesis or whether they are indicative of another nsP3 function that regulates the
  7 KB (1,096 words) - 08:37, 21 October 2021
• Part:BBa K3711081
  ...rates to produce acyl CoA thiolipids for subsequent reactions. Through the synthesis of these phenylpropane derivatives CoA lipids (e.g. p-gumaroyl CoA, feruloy ...". It exists widely in nature. ACC is a rate-limiting enzyme for ab initio synthesis of fatty acids, which catalyzes acetyl-CoA to malonyl-CoA, which eventually
  8 KB (1,337 words) - 13:38, 21 October 2021
• Part:BBa K3711082
  ...rates to produce acyl CoA thiolipids for subsequent reactions. Through the synthesis of these phenylpropane derivatives CoA lipids (e.g. p-gumaroyl CoA, feruloy ...". It exists widely in nature. ACC is a rate-limiting enzyme for ab initio synthesis of fatty acids, which catalyzes acetyl-CoA to malonyl-CoA, which eventually
  8 KB (1,333 words) - 13:38, 21 October 2021
• Part:BBa K3711084
  ...rates to produce acyl CoA thiolipids for subsequent reactions. Through the synthesis of these phenylpropane derivatives CoA lipids (e.g. p-gumaroyl CoA, feruloy ...". It exists widely in nature. ACC is a rate-limiting enzyme for ab initio synthesis of fatty acids, which catalyzes acetyl-CoA to malonyl-CoA, which eventually
  8 KB (1,329 words) - 13:41, 21 October 2021