User contributions
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- 20:40, 21 October 2019 (diff | hist) . . (+131) . . N File:T--Alma--tag gel1.png (A representative SDS PAGE gel for K2905018 in different hosts, alongside densitometery analysis performed with ImageStudio (Li-COR)) (current)
- 14:06, 21 October 2019 (diff | hist) . . (+836) . . Part:BBa K2905018
- 13:59, 21 October 2019 (diff | hist) . . (+47) . . N File:T--Alma--readthrough reporter1.png (Picture of bacteria from O/N growth on a plate.) (current)
- 13:41, 21 October 2019 (diff | hist) . . (+830) . . Part:BBa K2905016 (current)
- 13:34, 21 October 2019 (diff | hist) . . (+2,124) . . Part:BBa K204047 (current)
- 13:33, 21 October 2019 (diff | hist) . . (+1) . . m Part:BBa K574004 (→Measuring Expression: Alma 2019) (current)
- 13:33, 21 October 2019 (diff | hist) . . (+2,124) . . Part:BBa K574004
- 21:11, 19 October 2019 (diff | hist) . . (0) . . m Part:BBa K1692023 (→Measuring Expression: Alma 2019) (current)
- 21:11, 19 October 2019 (diff | hist) . . (0) . . Part:BBa K1692023
- 21:11, 19 October 2019 (diff | hist) . . (+6) . . m Part:BBa K1692023 (→Measuring Expression: Alma 2019)
- 16:17, 19 October 2019 (diff | hist) . . (+70) . . Part:BBa K1692023 (→Measuring Expression: Alma 2019)
- 16:13, 19 October 2019 (diff | hist) . . (+135) . . N File:T--Alma--AHLsynthaseFunction1.png (A graph comparing the Fluorescence and OD of a AHL biosensor when incubated with supernatant from AHL producing cells such as K1962014.) (current)
- 14:39, 19 October 2019 (diff | hist) . . (+230) . . N File:T--Alma--K515105 NH4.png (Expression and purification of sfGFP from K515105. Whole cell lysates were measured for sfGFP production - as a way to compare and assess the amount of sfGFP produced, this was run alongside an ammonium sulfate cut of the protein.) (current)
- 14:30, 19 October 2019 (diff | hist) . . (+423) . . N File:T--Alma--high pH graph1.png (Fluorescence/OD Units for RFP production of either the high pH sensor (K2905016) or a control (J04450). Cells were grown to mid-log in LB, then pelleted and transferred to M9 minimal media with pH adjusted to ~4.5 or left untouched at 7, and then grown...) (current)
- 16:03, 18 October 2019 (diff | hist) . . (+1,515) . . Part:BBa K2905005 (current)
- 16:03, 18 October 2019 (diff | hist) . . (+1,515) . . Part:BBa K2905003 (current)
- 16:02, 18 October 2019 (diff | hist) . . (+2) . . Part:BBa K2905001 (current)
- 16:02, 18 October 2019 (diff | hist) . . (-54) . . Part:BBa K2905001
- 16:02, 18 October 2019 (diff | hist) . . (+1,568) . . Part:BBa K2905001
- 13:46, 18 October 2019 (diff | hist) . . (+270) . . N File:T--Alma--sds page3.png (An SDS PAGE gel (stained with Coomaise, scanned with Li-COR odyssey Fc) of fractions from Ni-NTA purification of H6-tagged proteins from BL21 DE3 cells expressing MtbA or a negative control (empty vector). F = Flowthrough, W = Wash, E1&2 = Elution 1 an...) (current)
- 13:45, 18 October 2019 (diff | hist) . . (+241) . . N File:T--Alma--sds page2.png (An SDS PAGE gel (stained with Coomaise, scanned with Li-COR odyssey Fc) of fractions from Ni-NTA purification of H6-tagged proteins from BL21 DE3 cells expressing MttB or MttC. F = Flowthrough, W = Wash, E1&2 = Elution 1 and 2, respectively.) (current)
- 13:43, 18 October 2019 (diff | hist) . . (+177) . . N File:T--Alma--sds page1.png (An SDS PAGE gel (stained with Coomaise, scanned with Li-COR odyssey Fc) of total soluble and in-soluble protein from bacteria expressing MttB, MttC, MtbA, or a Negative Control.) (current)
- 03:07, 18 October 2019 (diff | hist) . . (+12) . . Part:BBa K515005 (→Characterization for Universal Golden Gate Cloning (Alma College))
- 03:07, 18 October 2019 (diff | hist) . . (+2,700) . . Part:BBa K515005
- 17:44, 14 October 2019 (diff | hist) . . (+93) . . N File:T--Alma--universalprimers2019 3.png (A table summarizing the results of various universal primer combinations in a PCR of K515005.) (current)
- 17:42, 14 October 2019 (diff | hist) . . (+79) . . N File:T--Alma--universalprimers2019 2.png (A diagram and a representative gel for PCR using universal golden gate primers.) (current)
- 00:57, 9 October 2019 (diff | hist) . . (+6) . . Part:BBa K1692023
- 00:56, 9 October 2019 (diff | hist) . . (+61) . . N File:T--Alma--AHLsynthaseGelDensity1.png (A graph showing the densitometry analysis of our SDS PAGE gel) (current)
- 00:56, 9 October 2019 (diff | hist) . . (+2,263) . . Part:BBa K1692023
- 00:51, 9 October 2019 (diff | hist) . . (+261) . . N File:T--Alma--AHLsynthaseExpression1.png (A representative gel image for expression of AHL synthases - specifically, parts K204047, K574004, and K1692023. This data shows that it is difficult to detect expression of these genes, despite the fact that another assay demonstrated that they are fu...) (current)
- 15:17, 24 June 2019 (diff | hist) . . (+32) . . Part:BBa K2905000:Design (current)
- 15:07, 24 June 2019 (diff | hist) . . (+124) . . Part:BBa K2905000 (current)
- 19:55, 19 November 2017 (diff | hist) . . (+48) . . m Part:BBa K1399010 (→Usage and Biology) (current)
- 19:55, 19 November 2017 (diff | hist) . . (+48) . . m Part:BBa K1399011 (current)
- 19:55, 19 November 2017 (diff | hist) . . (+48) . . m Part:BBa K1399012 (→Usage and Biology) (current)
- 19:55, 19 November 2017 (diff | hist) . . (-1) . . m Part:BBa K1399013 (→Usage and Biology) (current)
- 19:54, 19 November 2017 (diff | hist) . . (+49) . . Part:BBa K1399013
- 19:54, 19 November 2017 (diff | hist) . . (+2,303) . . Part:BBa K1399013:Experience (current)
- 19:54, 19 November 2017 (diff | hist) . . (+2,303) . . Part:BBa K1399012:Experience (current)
- 19:53, 19 November 2017 (diff | hist) . . (+2,303) . . Part:BBa K1399011:Experience (current)
- 19:53, 19 November 2017 (diff | hist) . . (+10) . . m Part:BBa K1399010:Experience (→Team Kingsborough NY 2017) (current)
- 19:52, 19 November 2017 (diff | hist) . . (+2,293) . . Part:BBa K1399010:Experience
- 19:41, 19 November 2017 (diff | hist) . . (+30) . . m Part:BBa J04450
- 19:39, 19 November 2017 (diff | hist) . . (-2) . . m Part:BBa J04450:Experience (→Team Kingsborough NY 2017)
- 19:39, 19 November 2017 (diff | hist) . . (+1,960) . . Part:BBa J04450:Experience (→Team Kingsborough NY 2017)
- 19:29, 19 November 2017 (diff | hist) . . (+90) . . N File:T--Kingsborough NY--RFP ssrA fig2.png (Quantitation of RFP J04450 grown with 1mM IPTG in either WT E. coli or ssrA::Kan-R E. coli) (current)
- 19:29, 19 November 2017 (diff | hist) . . (+157) . . N File:T--Kingsborough NY--RFP ssrA fig3.png (Bacteria with parts K1399010 - 13, or J04450, with or without ssrA (tmRNA ribosome rescue component) grown on LB High Salt (3% NaCl) with or without 1mM IPTG) (current)
- 19:28, 19 November 2017 (diff | hist) . . (+144) . . N File:T--Kingsborough NY--RFP ssrA fig1.png (Bacteria with parts K1399010 - 13, or J04450, with or without ssrA (tmRNA ribosome rescue component) grown on LB Miller with or without 1mM IPTG) (current)
- 19:27, 19 November 2017 (diff | hist) . . (+106) . . N File:T--Kingsborough NY--J04450 fig4.png (Bacteria with part J04450, with or without ssrA (tmRNA ribosome rescue component) grown on different media) (current)
- 19:03, 19 November 2017 (diff | hist) . . (+6) . . Part:BBa J04450:Experience (→Team Kingsborough NY 2017)
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