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• 02:43, 14 October 2022 Djcamenares (Talk | contribs) uploaded File:T--Alma--luxialign.png (Sequence alignment between LuxI and BjaI)
• 02:42, 14 October 2022 Djcamenares (Talk | contribs) uploaded File:T--Alma--luximutation.png (A summary of important residues in LuxI)
• 02:20, 14 October 2022 Djcamenares (Talk | contribs) uploaded File:T--Alma--luxi5w8g.png (A structural comparison between LuxI (homology model, based on 1RO5) and BjaI (crystalized))
• 01:39, 14 October 2022 Djcamenares (Talk | contribs) uploaded File:T--Alma--luximolstar.png (A picture of the SWISS Model structure of LuxI)
• 01:15, 14 October 2022 Djcamenares (Talk | contribs) uploaded File:T--Alma--dblinvplate.png (Plate comparing expression with double inverter)
• 01:13, 14 October 2022 Djcamenares (Talk | contribs) uploaded File:T--Alma--dblinvpigeon.png (A pigeon diagram of our double inverter)
• 01:05, 14 October 2022 Djcamenares (Talk | contribs) uploaded File:T--Alma--camp8pcn.png (Plasmid copy number of camp +8 mutation)
• 00:51, 14 October 2022 Djcamenares (Talk | contribs) uploaded File:T--Alma--a715gpcn.png (Plasmid copy number experiment for A715G)
• 00:24, 14 October 2022 Djcamenares (Talk | contribs) uploaded File:T--Alma--wtpcn.png (A figure showing data on plasmid copy number for J04450)
• 17:52, 13 October 2022 Djcamenares (Talk | contribs) uploaded File:T--Alma--ahlsyn.png (A diagram from the Brenda website showing synthesis of AHL)
• 02:52, 12 October 2022 Djcamenares (Talk | contribs) uploaded File:T--Alma--gel 006.png (Plasmid copy number determination by gel electrophoresis)
• 02:51, 12 October 2022 Djcamenares (Talk | contribs) uploaded File:T--Alma--gel 005.png (Plasmid copy number determination by gel electrophoresis)
• 02:50, 12 October 2022 Djcamenares (Talk | contribs) uploaded File:T--Alma--gel 004.png (Plasmid copy number determination for gel electrophoresis)
• 02:43, 12 October 2022 Djcamenares (Talk | contribs) uploaded File:T--Alma--pcnPlate.png (A plate of our different origin of replication mutants streaked onto an LB plate with Chloramphenicol.)
• 02:10, 12 October 2022 Djcamenares (Talk | contribs) uploaded File:T--Alma--ksGrowth.png (Growth curve of part K4166000 and associated controls (K4166001 and K4166014))
• 02:09, 12 October 2022 Djcamenares (Talk | contribs) uploaded File:KsGrowth.png (A growth curve of the kill switch part, K4166000, compared to controls K4166001 and K4166014, showing somewhat slower growth for the two replicates of the kill switch.)
• 02:07, 12 October 2022 Djcamenares (Talk | contribs) uploaded File:T--Alma--ksPlate.png (Growth (on a plate) of our Kill Switch part, K4166000 compared to controls K4166001 and K4166010)
• 02:06, 12 October 2022 Djcamenares (Talk | contribs) uploaded File:T--Alma--ksQPCR.png (qPCR data for the kill switch part K4166000)
• 01:50, 12 October 2022 Djcamenares (Talk | contribs) uploaded File:ModelVS5kra.png (Comparison of the rtER model versus human ER from PDB 5KRA)
• 01:12, 12 October 2022 Djcamenares (Talk | contribs) uploaded File:T--Alma--rtERhydro.png (A SWISS-MODEL of Rainbow Trout Estrogen Receptor, colored by hydrophobicity)
• 00:59, 12 October 2022 Djcamenares (Talk | contribs) uploaded File:T--Alma--hERhydro.png (Human estrogen receptor bound to DDT (PDB:5KRA), colored by hydrophobicity)
• 00:58, 12 October 2022 Djcamenares (Talk | contribs) uploaded File:T--Alma--3UUD5KRA.png (Pairwise alignment of 3UUD and 5KRA PDB structures (human estrogen receptor with estradiol or DDT))
• 00:56, 12 October 2022 Djcamenares (Talk | contribs) uploaded File:T--Alma--relativebindingER.png (Binding of human and rainbow trout estrogen receptor to different ligands, relative to the human protein binding estradiol)
• 01:41, 22 October 2021 Djcamenares (Talk | contribs) uploaded File:T--Alma--RtERsdsPAGE.png (SDS PAGE results for RtER steady state expression with 1mM IPTG)
• 01:30, 22 October 2021 Djcamenares (Talk | contribs) uploaded File:T--Alma--RtER qPCR.png (Amplification plot for RtER qPCR)
• 23:43, 21 October 2021 Djcamenares (Talk | contribs) uploaded File:T--Alma--plateATC.png (An assortment of plates with aTc filter discs)
• 13:27, 21 October 2021 Djcamenares (Talk | contribs) uploaded File:T--Alma--lowATC.png (Low concentrations of aTc vs. ZOI diameter)
• 13:26, 21 October 2021 Djcamenares (Talk | contribs) uploaded File:T--Alma--logATC.png (A log scale of ZOI diameter vs. aTc concentration)
• 16:04, 27 October 2020 Djcamenares (Talk | contribs) uploaded File:T--Alma--plot3.png (A qPCR plot of RFP (J04450) in E. coli)
• 16:03, 27 October 2020 Djcamenares (Talk | contribs) uploaded File:T--Alma--plot2.png (A qPCR result of K3445002 in E. coli)
• 16:02, 27 October 2020 Djcamenares (Talk | contribs) uploaded File:T--Alma--plot1.png (qPCR results of the plasmid in Vibrio)
• 16:02, 27 October 2020 Djcamenares (Talk | contribs) uploaded File:T--Alma--mhplate3.png (Transformation in Vibrio of pGGA (a pUC19 origin containing control plasmid, provided by NEB))
• 16:01, 27 October 2020 Djcamenares (Talk | contribs) uploaded File:T--Alma--mhplate2.png (Transformation in Vibrio of pSB1C3-BBa_K3445002; a C>>T reversion mutation to restore the original pUC19 origin sequence)
• 16:00, 27 October 2020 Djcamenares (Talk | contribs) uploaded File:T--Alma--mhplate1.png (Transformation (via electroporation) into Vibrio with pSB1C3-BBa_J04450)
• 14:24, 27 October 2020 Djcamenares (Talk | contribs) uploaded File:T--Alma--K3445001graph.png (A graph showing the fluorescence of K3445001)
• 14:23, 27 October 2020 Djcamenares (Talk | contribs) uploaded File:T--Alma--Gibson positiveControl.png (A positive control plate from Gibson assembly)
• 14:18, 27 October 2020 Djcamenares (Talk | contribs) uploaded File:T--Alma--plate3.png (Attempted assembly of K123003 and transformation. Possible cytotoxic effect?)
• 14:17, 27 October 2020 Djcamenares (Talk | contribs) uploaded File:T--Alma--i13521flz.png (Fluorescence of i13521 in different conditions)
• 14:07, 27 October 2020 Djcamenares (Talk | contribs) uploaded File:T--Alma--gel1.png (An SDS PAGE gel showing expression of J04450, I13521, K123002, and K3445001)
• 14:06, 27 October 2020 Djcamenares (Talk | contribs) uploaded File:T--Alma--plate2.png (A plate that shows i13521, J04450, or K3445001 in DH5a or HL2483)
• 14:05, 27 October 2020 Djcamenares (Talk | contribs) uploaded File:T--Alma--plate1.png (A streaked plate for characterization of I13521)
• 20:40, 21 October 2019 Djcamenares (Talk | contribs) uploaded File:T--Alma--tag gel1.png (A representative SDS PAGE gel for K2905018 in different hosts, alongside densitometery analysis performed with ImageStudio (Li-COR))
• 13:59, 21 October 2019 Djcamenares (Talk | contribs) uploaded File:T--Alma--readthrough reporter1.png (Picture of bacteria from O/N growth on a plate.)
• 16:13, 19 October 2019 Djcamenares (Talk | contribs) uploaded File:T--Alma--AHLsynthaseFunction1.png (A graph comparing the Fluorescence and OD of a AHL biosensor when incubated with supernatant from AHL producing cells such as K1962014.)
• 14:39, 19 October 2019 Djcamenares (Talk | contribs) uploaded File:T--Alma--K515105 NH4.png (Expression and purification of sfGFP from K515105. Whole cell lysates were measured for sfGFP production - as a way to compare and assess the amount of sfGFP produced, this was run alongside an ammonium sulfate cut of the protein.)
• 14:30, 19 October 2019 Djcamenares (Talk | contribs) uploaded File:T--Alma--high pH graph1.png (Fluorescence/OD Units for RFP production of either the high pH sensor (K2905016) or a control (J04450). Cells were grown to mid-log in LB, then pelleted and transferred to M9 minimal media with pH adjusted to ~4.5 or left untouched at 7, and then grown...)
• 13:46, 18 October 2019 Djcamenares (Talk | contribs) uploaded File:T--Alma--sds page3.png (An SDS PAGE gel (stained with Coomaise, scanned with Li-COR odyssey Fc) of fractions from Ni-NTA purification of H6-tagged proteins from BL21 DE3 cells expressing MtbA or a negative control (empty vector). F = Flowthrough, W = Wash, E1&2 = Elution 1 an...)
• 13:45, 18 October 2019 Djcamenares (Talk | contribs) uploaded File:T--Alma--sds page2.png (An SDS PAGE gel (stained with Coomaise, scanned with Li-COR odyssey Fc) of fractions from Ni-NTA purification of H6-tagged proteins from BL21 DE3 cells expressing MttB or MttC. F = Flowthrough, W = Wash, E1&2 = Elution 1 and 2, respectively.)
• 13:43, 18 October 2019 Djcamenares (Talk | contribs) uploaded File:T--Alma--sds page1.png (An SDS PAGE gel (stained with Coomaise, scanned with Li-COR odyssey Fc) of total soluble and in-soluble protein from bacteria expressing MttB, MttC, MtbA, or a Negative Control.)
• 17:44, 14 October 2019 Djcamenares (Talk | contribs) uploaded File:T--Alma--universalprimers2019 3.png (A table summarizing the results of various universal primer combinations in a PCR of K515005.)

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