Regulatory
EGSH
Part:BBa_K415507
Designed by: Laura Deming, Joy Jiao, Adrian Slusarczyk Group: iGEM10_MIT (2010-10-27)
pEGSH L4R1 MammoBlock Entry Vector
Schematic: Response of K415507 to the addition of PonS, in the presence of VgEcR and RxR.L4R1 MammoBlock entry vector. EGSH is an inducible promoter. In the presence of the proteins retinoid X receptor (RxR) and ecdysone receptors (VgEcR), the addition of ponasterone S (PonS) leads to complex formation of RxR, VgEcR, and PonS. This activated complex is then capable of inducing the EGSH promoter and allows for dramatic upregulation in transcriptional activity.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 6
Illegal EcoRI site found at 671
Illegal PstI site found at 485 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 6
Illegal EcoRI site found at 671
Illegal PstI site found at 485 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 6
Illegal EcoRI site found at 671
Illegal BglII site found at 115
Illegal BamHI site found at 663 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 6
Illegal EcoRI site found at 671
Illegal PstI site found at 485 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 6
Illegal EcoRI site found at 671
Illegal PstI site found at 485 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 646
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Categories
Parameters
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