Regulatory
EGSH

Part:BBa_K415507

Designed by: Laura Deming, Joy Jiao, Adrian Slusarczyk   Group: iGEM10_MIT   (2010-10-27)
Revision as of 18:34, 28 October 2010 by Ldeming (Talk | contribs)

pEGSH L4R1 MammoBlock Entry Vector

L4R1 MammoBlock entry vector containing the EGSH ecdysone-inducible promoter. This promoter is controlled by a heterodimer between the VgEcR and RxR proteins.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 671
    Illegal PstI site found at 485
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 671
    Illegal PstI site found at 485
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 671
    Illegal BglII site found at 115
    Illegal BamHI site found at 663
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 671
    Illegal PstI site found at 485
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 671
    Illegal PstI site found at 485
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 646


[edit]
Categories
Parameters
None